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为研究大豆花青素合成途径关键酶的基因标记和结构信息,利用大豆基因组和染色体标记数据,对16个花青素合成关键酶基因(苯丙氨酸解氨酶(PAL)、查尔酮合酶(CHS,包括9个成员)、黄烷酮-3-羟化酶(F3H)、苯基苯乙烯酮黄烷酮异构酶(CHI)、二氢黄酮醇还原酶(DFR)和4-香豆酰CoA连接酶(4CL),进行基因遗传图和物理图定位和基因结构分析。结果表明:16个基因分别定位在A1、A2、B1、B2、D1a、D1b、D2、I、K、O等10个连锁群上,并获得了基因所在序列两侧标记。利用大豆的cDNA和gDNA序列信息,获得了16个基因的结构,外显子数目1～7个,内含子数目0～6个,其中PAL、DFR2、GmCHS7是单外显子基因,4CL、CHI、F3H、GmCHS1、GmCHS5、GmCHS8有1个内含子,DFR1、GmCHS2、GmCHS3、GmCHS6有2个内含子,GmCHS4、GmCHS9有3个内含子,GmIRCHS则有6个内含子。
In order to study the genetic markers and structural information of key enzymes involved in the anthocyanin biosynthesis pathway, 16 genes encoding key anthocyanin biosynthesis genes, PAL, Chalcone, were analyzed using soybean genomic and chromosomal marker data. (CHS, including 9 members), flavanone-3-hydroxylase (F3H), phenylstyrylflavanone isomerase (CHI), dihydroflavonol reductase (DFR) and 4 The results showed that 16 genes were located in A1, A2, B1, B2, D1a, D1b, D2, I, K , O and other 10 linkage groups, and obtained on both sides of the sequence of the gene marker using soybean cDNA and gDNA sequence information obtained 16 gene structure, the number of exons 1 to 7, the number of introns 0 ~ 6, of which PAL, DFR2 and GmCHS7 are single exon genes. There are 1 intron in 4CL, CHI, F3H, GmCHS1, GmCHS5 and GmCHS8, 2 introns in DFR1, GmCHS2, GmCHS3 and GmCHS6, , GmCHS9 has 3 introns, GmIRCHS has 6 introns.