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目的 观察安宫牛黄丸的抗肿瘤作用,并探索其可能的作用机制.方法 安宫牛黄丸9,30,90,300和900 mg·L-1分别与人胃癌MGC-803和人肝癌BEL-7402细胞孵育24,48和72 h.采用噻唑蓝[3-(4,5-二甲基噻唑-2-基)-5(3-羧基甲基苯基)-2-(4-磺酸苯基)-2H-四氮唑)细胞毒实验MTS法和克隆实验观察安宫牛黄丸对MGC-803和BEL-7400细胞增殖的影响；应用Hoechst 33258/PI染色和流式细胞仪检测细胞凋亡；荧光分光光度计检测线粒体膜电位.结果 安宫牛黄丸具有浓度依赖性地抑制肿瘤细胞增殖的作用.MTS法测定结果表明,安宫牛黄丸作用48和72 h对MGC-803细胞增殖具有抑制作用,浓度-效应相关系数分别为0.996(P =0.000)和0.756(P =0.004)；与BEL-7402细胞作用48和72 h的浓度-效应相关系数分别为0.732(P =0.030)和0.700(P =0.037).细胞克隆实验结果表明,安宫牛黄丸作用24h可浓度依赖性地抑制MGC-803细胞(r=0.914,P=0.011)和BEL-7402细胞(r=0.871,P=0.024)克隆形成.Hoechst 33258/PI染色和流式细胞仪检测结果表明,安宫牛黄丸900 mg·L-1作用24h可诱导MGC-803和BEL-7402细胞凋亡,细胞凋亡百分率分别达27.2％和19.7％.安宫牛黄丸900 mg· L-1作用后连续检测3 min,MGC-803和BEL-7402细胞的线粒体膜电位比对照组分别下降15.9％和15.0％.结论 安宫牛黄丸具有抑制肿瘤细胞增殖的作用,其作用机制与诱导细胞凋亡和降低肿瘤细胞线粒体膜电位有关.“,”OBJECTIVE To validate the anticancer effect of Angong Niuhuang pill (AGNH) and pinpoint associated molecular mechanisms using human cancer cells.METHODS Human MGC-803 gastric carcinoma and human BEL-7402 hepatocarcinoma cells were incubated with AGNH 9,30,90,300 and 900 mg·L-1 for 24,48and 72 h,respectively.Cell viability was detected with 3-(4,5-dimethylthiazol-2-yl) -5-( 3-carboxymethoxyphe-nyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and colony formation assay.Apoptosis was measured with flow cytometry and Hoechst 33258/PI staining.Change in mitochondrial membrane potential (△qψ) was detected by spectrofluorophotometer.RESULTS AGNH inhibited MGC-803 cell proliferation ( for 48 h,r =0.996,P =0.002; for72 h,r=0.756,P=0.024 ) and BEL-7402 cells (for 48 h,r =0.732,P=0.030; for72 h,r=0.702,P =0.037) in a concentration-dependent manner,as showed by MTS assay.AGNH inhibited colony formation on MGC-803 cells (r =0.914,P =0.011 ) and BEL-7402 cells (r =0.871,P =0.024) in a concentration-dependent manner for 24 h.Hoechst 33258/PI staining and flow cytometry assay showed that AGNH 900 mg·L-1 for 24 h induced apoptosis of MGC-803 and BEL-7402 cells,and the apoptosis rate was 27.2％ and 19.7％,respectively.Compared with normal control group,AGNH 900 mg·L-1 for 3 min decreased the mitochondrial membrane potential of MGC-803 and BEL-7402 cells to 15.9％ and 15.0％ of control group.CONCLUSION AGNH inhibits proliferation of human cancer cells.Apoptosis and depolarization of mitochondrial transmembrane potential are probablly its mechanism.