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目的:探讨内皮型一氧化氮合酶(eNOS)基因894G/T多态性与原发性高血压(EH)合并脑梗塞(CI)的关系。方法:应用聚合酶链反应限制性片段长度多态性方法检测湖北地区汉族74例健康者(NT组)、103例原发性高血压无合并症者(EH组)及70例原发性高血压合并脑梗塞者(EH-CI组)的eNOS基因型;生化技术测定其血脂、一氧化氮代谢物(NOM)水平。结果:EH组及EH-CI组患者的T等位基因频率分别为0.224和0.321,均显著高于NT组(P<0.05);且两者之间的T等位基因频率差异显著性(P<0.05);EH-CI组中,GT+TT基因型者的舒张压显著高于GG基因型者(P<0.05),而NOM显著低于GG基因型者。结论:eNOS基因894位G/T多态性可能与汉族高血压病患者伴脑梗塞有关,该位点多态性可能使T等位基因携带者NOM减少,进而参与EH-CI发病。
Objective: To investigate the relationship between polymorphism of endothelial nitric oxide synthase (eNOS) gene 894G / T and essential hypertension (EH) complicated with cerebral infarction (CI). Methods: A total of 74 healthy subjects (NT group), 103 hypertensive patients with no comorbid hypertension (EH group) and 70 patients with primary hypermetabolism were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) The eNOS genotypes in the blood pressure patients with cerebral infarction (EH-CI) were measured. The levels of lipids and nitric oxide metabolites (NOM) were determined by biochemical techniques. Results: The frequencies of T allele in EH group and EH-CI group were 0.224 and 0.321, respectively, which were significantly higher than those in NT group (P <0.05). The frequencies of T allele were significantly different between EH group and EH-CI group <0.05). In the EH-CI group, the diastolic blood pressure of the GT + TT genotype was significantly higher than that of the GG genotype (P <0.05), but NOM was significantly lower than that of the GG genotype. CONCLUSION: The 894-bp G / T polymorphism of eNOS gene may be associated with cerebral infarction in patients with Hypertension in Han nationality. Polymorphism at this locus may reduce NOM in T-allele carriers and further participate in the pathogenesis of EH-CI.