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目的:应用人发角蛋白神经导管植人兔胫神经缺损部分,诱导神经再生过程中不同时期泛素(ｕｂｉｑｕｉｔｉｎ)的表达和分布。方法:免疫组织化学法。结果:术后４８ｄ,缝合线之间变性组织及其周围神经组织的雪旺细胞胞质和胞核中泛素免疫组化染色呈棕黄色阳性反应。术后９９ｄ,泛素在人发角蛋白周围的组织中免疫组化染色呈深棕色强阳性反应,在其它新生神经组织分布稀疏,免疫组化染色呈浅黄色弱阳性。术后９９ｄ较成熟的神经组织中无泛素阳性着色,而新生的神经组织呈棕黄色阳性反应。结论:人发角蛋白及缝合线之间变性组织的异常蛋白的降解主要由泛素承担。在新生神经组织中大量泛素参与蛋白质和变性神经组织的降解,而在趋于成熟的神经组织中因降解的蛋白质量的减少,参与降解的泛素量也减少。
OBJECTIVE: To investigate the expression and distribution of ubiquitin during different stages of neural regeneration induced by human hair keratin neurotransplantation in rabbits. Methods: Immunohistochemistry. RESULTS: On day 48 after operation, ubiquitin immunohistochemical staining of the Schwann cell cytoplasm and nucleus in the degenerative tissues and their peripheral nerve tissues showed a positive brown-yellow staining. At 99 days after operation, ubiquitin showed a dark brown strong positive reaction in the tissues around the human hair keratin. The distribution of ubiquitin was sparse in other newborn nerves and was weakly positive in the light yellow by immunohistochemistry. After 99 days more mature neural tissue without ubiquitin-positive staining, and nascent neural tissue was brown-positive reaction. Conclusion: Degradation of abnormal proteins in degenerative tissues between human hair keratin and sutures is mainly undertaken by ubiquitin. A large number of ubiquitin are involved in the degradation of protein and denatured nerve tissue in nascent neural tissue, while the amount of ubiquitin involved in degradation also decreases in the mature nervous tissue due to the decreased amount of degraded protein.