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目的:评价序贯高速离心消除脂血对常规生化项目检测干扰的效果。方法 :通过将脂血血浆于试管内直立过夜获得上层脂质,与澄清血浆(原始血浆)按一定比例混合制造脂血模型,脂血模型以序贯高速离心后测20项生化指标,并与原始血浆的生化指标结果比较。结果:6.55 mmol/L三酰甘油(triglyceride,TG)脂血有Cl~-、Mg~(2+)、P~(3-)、谷丙转氨酶(alanine aminotransferase,ALT)、总蛋白(total protein,TP)、白蛋白(albumin,ALB)、总胆红素(total bilirubin,TBIL)、谷氨酰转肽酶(glutamyl transpeptidase,GGT)、碱性磷酸酶(alkaline phosphatase,ALP)、乳酸脱氢酶(lactate dehydrogenase,LDH)、淀粉酶(amylase,AMY)、尿素氮(blood urea nitrogen,BUN)的偏倚超过中国临床生物化学常规检验项目分析质量标准,10.48 mmol/L TG脂血除上述指标外,还有肌酸激酶(creatine kinase,CK)、谷草转氨酶(aspartate transaminase,AST)的偏倚超过国家规定的标准;序贯高速离心处理后,Mg~(2+)、P~(3-)、ALT、AST、ALB、ALP、LDH、AMY偏倚不大于国家规定的标准,Cl~-、TP、TBIL、GGT、CK、BUN大于国家规定的标准。结论 :本实验室的条件下序贯高速离心处理能有效去除脂质对Mg~(2+)、P~(3-)、ALT、AST、ALB、ALP、LDH、AMY检测的影响。
OBJECTIVE: To evaluate the efficacy of sequential high-speed centrifugation in the elimination of lipids on routine biochemical assays. Methods: Lipid blood was obtained by mixing lipid plasma in the test tube for overnight, and then mixed with the clarified plasma (original plasma). The lipid profile was determined by sequential high speed centrifugation. Biochemical indicators of the original plasma results comparison. Results: The contents of Cl ~ -, Mg ~ (2 +), P ~ (3-), alanine aminotransferase (ALT) and total protein in 6.55 mmol / L triglyceride (TG) (TP), albumin (ALB), total bilirubin (TBIL), glutamyl transpeptidase (GGT), alkaline phosphatase (ALP) Bias of lactate dehydrogenase (LDH), amylase (AMY) and blood urea nitrogen (BUN) surpassed the quality standard of routine clinical biochemical routine test in China. In addition to the above indexes, 10.48 mmol / L TG lipids , And the bias of creatine kinase (CK) and aspartate transaminase (AST) exceeded the national standard. After sequential high-speed centrifugation, Mg 2+, P 3-, The bias of ALT, AST, ALB, ALP, LDH and AMY was not greater than the national standard, and Cl ~ -, TP, TBIL, GGT, CK and BUN were greater than the national standards. CONCLUSION: Sequential high-speed centrifugation under this laboratory condition can effectively remove the effect of lipid on the detection of Mg 2+, P 3, ALT, AST, ALB, ALP, LDH and AMY.