通过花药离体培养,采用变动了的B_5培养基,成功地诱导出大豆花粉愈伤组织,并分化出完整的大豆幼苗。经根尖染色体检查,2n=20,证明是由单倍性花粉发育而来的。 花粉发育时期以单核早、中期较为合适。 离体培养大豆花药,单核花粉可以通过不均等分裂和均等分裂由营养性质的核分裂成两个细胞,它们继续分裂形成多细胞球,多细胞球进一步发育成为外形不规则的花粉愈伤组织块,在适宜的条件下,进而分化出单倍体幼苗。 Through in vitro culture of the anther, we successfully induced the soybean pollen callus and transformed the intact soybean seedlings with the changed B_5 medium. The apical chromosome examination, 2n = 20, proved to be derived from haploid pollen. Pollen development period to mononuclear early, mid-term more appropriate. In vitro culture of soybean anthers, mononuclear pollen can be divided into two cells from the nourishing nucleus by unequal division and equal division, and they continue to divide to form multicellular spheroids. The multicellular spheroids further develop into irregularly shaped pollen callus pieces , Under appropriate conditions, and then differentiated haploid seedlings.