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本文报告采用三种离体培养鼠疟原虫动合子的方法。 1.分离培养法形成的动合子数量最多,离心培养法次之,直接培养法最少。三种方法获得的动合子大小范围基本相似,形态正常,呈典型的香蕉样。 2.三种方法在7小时均出现少量动合子,动合子数量高峰时间,直接培养法和离心培养法为12小时,分离培养法为42小时。7～12小时离心培养法和分离培养法上升幅度较大,达高峰后下降的也较缓慢,直接培养法开始上升幅度不大,下降的却较快。 3.动合子形成的数量与配子体数目一般成正比关系。离心培养法与分离培养法配子体明显浓集,形成动合子数量明显增加。 4.采用感染鼠疟的2只、3只或4只鼠血混合,取得较多的鼠血作分离培养,为今后大量培养提供可能性。
This paper reports the use of three methods of in vitro culture of P. murine parasite. 1. Isolated culture method to form the largest number of dynamic zygotes, followed by centrifugal culture method, direct culture method at least. The three methods to obtain the size of the moving zygote basically the same, the normal form, showed a typical banana-like. 2. The three methods in a small amount of 7 hours in a small number of moving zygotes, the peak number of moving zygotes, direct culture and centrifugation culture for 12 hours, culture method for 42 hours. After centrifugation and isolation culture for 7 ~ 12 hours, the increase rate was larger and reached a peak and then decreased more slowly. However, the direct culture method did not start to increase much but decreased rapidly. 3. The number of zygotes formed is generally proportional to the number of gametophytes. Centrifugal culture method and separation culture method gametophyte significantly concentrated to form a significant increase in the number of mover. 4. Using murine malaria infected 2, 3 or 4 rat blood mixed to obtain more rat blood for isolation and culture, provide the possibility for mass culture in the future.