论文部分内容阅读
目的探讨芪参益气滴丸对心肌梗死后左室结构及心肌细胞的作用及可能的机制。方法成功建立大鼠心肌梗死模型后随机分为对照组、药物组,各15只,另设假手术组15只。药物组给予38m g/(kg.d)-1芪参益气滴丸灌胃;对照组和假手术组给等量生理盐水。8周后测定血流动力学、超声心动图、心肌梗死面积、心肌及血浆血管紧张素Ⅱ(A ngⅡ)水平、心肌细胞凋亡指数(A I)、心肌细胞B cl-2和B ax蛋白表达等指标,并用光镜和透射电镜作形态学观察。结果对照组左室重量指数(LVM I)、室间隔厚度(IV ST)、左室后壁厚度(LVPW T)、左室舒张末压(LVEDP)、心肌梗死面积等均大于假手术组(P<0.05或P<0.01);药物组LVM I、IV ST、LVEDP、梗死面积、心肌及血浆A ngⅡ、A I等均低于对照组(P<0.05或P<0.01),心肌B cl-2蛋白阳性表达高于对照组(P<0.05),B ax蛋白阳性表达低于对照组(P<0.05)。光镜和电镜观察显示药物组心肌损害程度较对照组明显减轻。结论应用芪参益气滴丸可减轻大鼠心肌梗死后左室重构并改善血流动力学指标,其作用可能与降低A ngⅡ水平,调节心肌B cl-2和B ax蛋白表达,减轻心肌细胞凋亡有关。
Objective To investigate the effect of Qi-shen Yi-qi drop pill on left ventricular structure and myocardial cell after myocardial infarction and its possible mechanism. Methods The model of myocardial infarction in rats was successfully established. The rats were randomly divided into control group and drug group, 15 rats in each group, and 15 rats in sham operation group. The drug group was given Astragalus membranaceus with 38m g / (kg · d -1) dropping pills; the control group and the sham operation group were given the same amount of saline. After 8 weeks, hemodynamics, echocardiography, myocardial infarct size, myocardial and plasma levels of angiotensin Ⅱ (A ngⅡ), cardiomyocyte apoptosis index (AI), cardiomyocytes Bcl-2 and Bax protein expression And other indicators, and light microscopy and transmission electron microscopy for morphological observation. Results The left ventricular mass index (LVVM), IV ST, LVPW T, LVEDP and MI in the control group were significantly higher than those in the sham operation group (P <0.05 or P <0.01). The levels of LVM I, IV ST, LVEDP, infarct size, A ngⅡ and AI in myocardium and plasma were lower in the drug group than those in the control group (P <0.05 or P <0.01) The positive expression of Bax protein was lower than that of the control group (P <0.05). Light and electron microscopy showed that the degree of myocardial damage in the drug group was significantly reduced compared with the control group. Conclusion Qishenyiqi dripping pill can relieve left ventricular remodeling and improve hemodynamics after myocardial infarction in rats, which may be related to the decrease of A ngⅡ level, regulation of Bcl-2 and Bax protein expression, alleviation of cardiac muscle Apoptosis related.