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目的:研究镉导致核苷酸切除修复基因XPC在HEK293细胞中的表达变化,探讨XPC基因在镉导致细胞DNA突变种的作用。方法:离体培养的人胚肾293细胞以20umol/L(低剂量组)和40umol/L(高剂量组)分别氯化镉处理24小时,Real-timePCR检测处理前后XPC的表达变化情况。结果:两组中,氯化镉处理前后XPC的表达明显升高(P<0.01);高剂量组中XPC的表达与低剂量组中XPC的表达没有明显差别(P>0.05)。结论:高表达的XPC、XPF在镉导致的DNA突变中起着重要的修复作用,核苷酸切除修复机制对镉造成的DNA损伤具有一定的修复作用。
OBJECTIVE: To study the expression of nucleotide excision repair gene XPC in HEK293 cells induced by cadmium, and to explore the role of XPC gene in the mutation of DNA caused by cadmium. Methods: Human embryonic kidney 293 cells cultured in vitro were treated with cadmium chloride (20umol / L (low dose) and 40umol / L (high dose) for 24 hours respectively. The expression of XPC was detected by Real-time PCR. Results: In both groups, the expression of XPC was significantly increased before and after treatment with cadmium chloride (P <0.01). The expression of XPC in high dose group was not significantly different from that in low dose group (P> 0.05). CONCLUSION: Highly expressed XPC and XPF play an important role in the repair of DNA-induced DNA damage. The mechanism of nucleotide excision and repair has some effects on DNA damage caused by cadmium.