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了解乙型肝炎患者补体介导 ＨＢｓＡｇ特异性免疫复合物溶解能力（ＣＭＳＣ）及补体介导免疫沉淀 的抑制作用（ＩＩＰＣ）两种新功能低下在乙型肝炎发病中的作用。方法：采用ＰＥＧ沉淀法测定患者血清循环免疫复 合物（ＣＩＣ），以ＨＢｓＡｇ和抗－ＨＢｓ制备特异性免疫复合物（ＩＣ），加入补体（血清）抑制其沉淀，或使ＩＣ裂解并定量测 定上清中的ＨＢｓＡｇ，确定ＣＭＳＣ及ＩＩＰＣ功能，同时用单向琼脂扩散法测定Ｃ３、Ｃ４。结果：患者组ＣＩＣ检出阳性率为 ４５．０％～８２．６％，均显著高于正常组（Ｐ＜０．０５～０．００１）；正常组ＣＭＳＣ后上清液ＨＢｓＡｇ为０．５８±０．１３ｎｇ，各种乙肝 组均明显低于正常对照组（Ｐ＜０．０５～０．００１）；ＩＩＰＣ后上清中ＨＢｓＡｇ浓度为０．４５±０．１８ｎｇ，急性肝炎、慢活肝、重症 肝炎均显著降低（Ｐ＜０．００１），肝硬化组亦明显降低（Ｐ＜０．０５）；Ｃ３、Ｃ４在患者组亦有不同程度的降低。结论：乙型 肝炎患者血清中存在特异性ＩＣ，患者ＣＭＳＣ及ＩＩＰＣ明显降低在乙型肝炎的发病中有一定作用，补体成分的减少与 ＣＭＳＣ和ＩＩＰＣ的降低有一定关系。
To understand the role of complement-mediated HBsAg-specific immunosuppressive capacity (CMSC) and complement-mediated immunoprecipitation (IIPC) in patients with hepatitis B in the pathogenesis of hepatitis B. Methods: Serum circulating immune complexes (CICs) were determined by PEG precipitation method. Specific immune complexes (ICs) were prepared by HBsAg and anti-HBs. Complement (serum) was added to inhibit the precipitation of ICs or ICs were lysed and quantified Qing HBsAg, CMSC and IIPC to determine the function, while using one-way agar diffusion method for the determination of C3, C4. Results: The positive rate of CIC in patients was 45.0% ~ 82.6%, which were significantly higher than those in normal group (P <0.05 ~ 0.001). The HBsAg of supernatant in CMSC was 0.58 ± 0.13ng, all kinds of hepatitis B group were significantly lower than the normal control group (P <0.05 ~ 0.001); HBsAg concentration in the supernatant after IIPC was 0.45 ± 0.18ng, acute hepatitis, slow living liver , Severe hepatitis (P <0.001), and liver cirrhosis group (P <0.05). Patients with C3 and C4 also had different degrees of reduction. Conclusion: There are specific ICs in the serum of patients with hepatitis B, and the significant decrease of CMSC and IIPC in patients with hepatitis B may play a role in the pathogenesis of hepatitis B. The decrease of complement components has something to do with the decrease of CMSC and IIPC.