目的对水产品牡蛎污染耐药性细菌进行调查,并检测其携带整合子类型,为细菌耐药机制研究提供依据。方法利用抗性培养基平板筛选牡蛎标本中的耐药性细菌,用K-B法进行药敏试验,PCR法扩增Ⅰ型、Ⅱ型和Ⅲ型整合酶基因。结果 34份牡蛎标本分离出76株耐药株,其中超过90%的分离菌对四环素、氨苄西林耐药,诺氟沙星、左氧氟沙星、复方新诺明、环丙沙星、头孢唑啉和氯霉素耐药>50%,有2株对耐亚胺培南细菌。整合酶基因检测结果显示80.26%耐药细菌携带Ⅰ型整合子,其中6株同时携带Ⅱ型整合子,未检测到Ⅲ型整合子。所有携带整合子的耐药细菌是多重耐药,不携带整合子耐药细菌中多重耐药占60%。结论牡蛎污染细菌的耐药性严重,且多重耐药与携带整合子有关,携带整合子细菌更易产生多重耐药,提示整合子可作为食品分离菌的耐药性检测靶标。 OBJECTIVE: To investigate oyster-contaminated bacteria in aquatic products and to detect the types of integron that they carry, so as to provide basis for the study of bacterial resistance mechanism. Methods Resistant culture medium was used to screen drug-resistant bacteria in oyster samples. K-B method was used for drug sensitivity test. The type I, type II and type III integrase genes were amplified by PCR. Results A total of 76 drug-resistant strains were isolated from 34 oyster samples. Over 90% of isolates were resistant to tetracycline, ampicillin, norfloxacin, levofloxacin, cotrimoxazole, ciprofloxacin, Mycotoxin resistance> 50%, 2 strains of imipenem-resistant bacteria. Integrase gene test results showed that 80.26% of drug-resistant bacteria carried type I integron, of which 6 also carried type II integron, but no type III integron was detected. All resistant bacteria carrying the integron were multidrug resistant and 60% resistant to multi-drug resistant bacteria without integron. Conclusion The oyster-contaminated bacteria are highly resistant and the multi-drug resistance is associated with carrying the integron. The multi-drug resistance is more likely to occur with the integrant sub-bacteria, suggesting that the integron can be used as a drug-resistant detection target for food isolates.