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目的研究胰岛素样生长因子结合蛋白(IGFBP)在肝纤维化和肝硬化患者肝组织中的表达、分布和意义。方法对18例肝纤维化、18例肝硬化患者肝组织和6例对照组(为手术切除的肝血管瘤外的正常肝组织)标本分别进行HE染色、VG染色,以及TGF-β1、TGFRΙ、胶原纤维、IGFBP-2,-6,-7及CD14的免疫组织化学染色,并将其通过图像分析系统进行半定量分析和相关分析。结果对照组正常肝组织IGFBP-2,-6,-7的表达表现为极少数肝细胞、中央静脉和胆管上皮细胞有阳性染色,在肝纤维化组和肝硬化组的表达主要见于肝窦内和纤维间隔。并且IGFBP-2,-6,-7在对照组正常肝组织、肝纤维化组和肝硬化组肝组织的表达逐渐增高,组间差异有统计学意义(P<0.05)。定量分析肝组织IGFBP-2,-6,-7的表达分别与肝组织胶原纤维、TGF-β1、TGFRΙ、CD14的表达呈正相关。结论IGFBP-2,-6,-7在人肝纤维化的发生发展中可能起重要作用。
Objective To study the expression, distribution and significance of insulin-like growth factor binding protein (IGFBP) in liver tissues of patients with liver fibrosis and cirrhosis. Methods Eighteen cases of hepatic fibrosis, 18 cases of liver cirrhosis and 6 cases of control group, which were resected normal hepatic hemangiomas, were stained with HE, VG and TGF-β1, TGFR1, Collagen fibers, IGFBP-2, -6, -7 and CD14 immunohistochemical staining and image analysis system for semi-quantitative analysis and correlation analysis. Results The expression of IGFBP-2, -6, -7 in the normal liver tissues of the control group showed positive staining in very few hepatocytes, central veins and bile duct epithelial cells. The expression of IGFBP-2, And fiber spacing. And the expression of IGFBP-2, -6, -7 in the control group normal liver tissue, liver fibrosis group and cirrhosis group liver gradually increased, the difference between the groups was statistically significant (P <0.05). Quantitative analysis of the expression of IGFBP-2, -6, -7 in liver tissue was positively correlated with the expression of collagen fibers, TGF-β1, TGFR1 and CD14 in liver tissue. Conclusion IGFBP-2, -6, -7 may play an important role in the development of human liver fibrosis.