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目的从正常成人脑海马分离鉴定多潜能神经前体细胞并探讨体外培养条件。方法材料取自8例非神经系统疾病的死亡者。分离正常成人脑海马细胞,在培养基中添加生长因子体外培养。通过细胞培养成球和BrdU染色鉴定培养细胞的增殖能力。利用免疫细胞化学三标染色来鉴定分化神经细胞的表型。结果从正常人海马分离的细胞培养2周时,开始增殖成簇,致一个月时,形成细胞球, 这些细胞能与BrdU结合,并能在体外分化成神经系统不同谱系的细胞。结论与在啮齿类动物和猴的研究中结果一致,从正常成人脑海马区分离的细胞是多潜能神经前体细胞。
Objective To isolate and identify multipotent neural precursor cells from normal adult hippocampus and to investigate the culture conditions in vitro. Methods Materials were taken from 8 deaths from non-neurological diseases. Normal adult hippocampal cells were isolated and cultured in vitro with growth factors. Cultured cells were identified for proliferative capacity by cell culture into pellets and BrdU staining. Three-stained immunocytochemistry was used to identify the phenotype of differentiated nerve cells. Results When cells isolated from normal hippocampus cultured for 2 weeks, they began to proliferate into clusters. After a month, they formed spheres. These cells could bind to BrdU and differentiate into cells of different lineages in the nervous system in vitro. Conclusions Consistent with studies in rodents and monkeys, cells isolated from normal adult brain hippocampus are pluripotent neural progenitor cells.