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针对小麦赤霉病抗源苏麦3号构建的两个小麦重组自交系遗传群体苏麦3号/Alondra和苏麦3号/安农8455,采用单花接种、表土接种及自然发病3种不同的接种方法进行小麦赤霉病抗性接种鉴定,并根据苏麦3号赤霉病抗性主效QTL的连锁分子标记Xgwm493和Xgwm533.1分别对群体进行抗性连锁分析.检测结果表明,在温室单花接种所获得的鉴定数据中,标记的赤霉病抗性连锁效应最高,P值分别小于0.0001,抗性鉴定结果最为准确.研究表明,对小麦赤霉病这种由数量性状控制,受外界环境影响较大的真菌病害进行抗扩展性的遗传研究,应采用控温控湿条件下的单花滴注接种鉴定方法最为合适.
Two wheat recombinant inbred lines, Sumai 3 / Alondra and Sumai 3 / Anyong 8455, were constructed from three wheat cultivars resistant to wheat scab (Sumai 3), including single flower inoculation, topsoil inoculation and three natural occurrences Different inoculation methods were used to identify the Fusarium graminearum resistance inoculation, and the linkage analysis of the population was carried out based on the linkage markers Xgwm493 and Xgwm533.1 of QTL for main resistance to Fusarium head blight Sumai 3. The test results showed that, Among the identification data obtained by single flower inoculation in greenhouse, the linkage of the scab resistance was the highest with the P values less than 0.0001, respectively, and the most accurate identification of the resistance.The results showed that the scab resistance was controlled by the quantitative trait , By the external environment of the fungal disease greater resistance to the expansion of genetic research should be used under the conditions of controlled temperature and humidity single flower inoculation identification method is most appropriate.