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目的:研制肝靶向牛血清白蛋白(bovine serum albumin,BSA)脂质体,并研究其理化性质,为蛋白质药物的肝靶向给药提供一种新模型。方法:首先采用逆相蒸发法制备BSA负电荷脂质体,再用O-羧甲基-N-乳糖酰化壳聚糖(O-carboxymethyl N-galactosylated chitosan,Gal-CMCS)修饰,制备肝靶向BSA脂质体,并考察脂质体的形态、粒径、Zeta电位、包封率及稳定性等理化性质。结果:制得的肝靶向BSA脂质体在电镜下呈类圆形,粒径分布均匀,平均粒径为(284.1±5.41)nm,BSA包封率为(34.21±1.73)%,Zeta电位为(+27.1±1.17)mV,4℃下放置3个月粒径和包封率无明显变化。结论:成功制备了肝靶向BSA脂质体,其具有良好的稳定性和较高的药物包封率,可作为蛋白质药物肝靶向给药的一种新模型。
OBJECTIVE: To develop a liver targeting bovine serum albumin (BSA) liposome and study its physicochemical properties to provide a new model for the targeted liver drug delivery of protein drugs. Methods: BSA negatively charged liposomes were prepared by reversed-phase evaporation and then modified with O-carboxymethyl N-galactosylated chitosan (Gal-CMCS) to prepare liver targets To BSA liposomes and liposomes morphology, particle size, Zeta potential, entrapment efficiency and stability and other physical and chemical properties. Results: The prepared liver-targeting BSA liposomes showed a round shape and a uniform size distribution with an average diameter of (284.1 ± 5.41) nm. The BSA encapsulation efficiency was (34.21 ± 1.73)%, the zeta potential (+ 27.1 ± 1.17) mV, there was no significant change in particle size and encapsulation efficiency after being placed at 4 ℃ for 3 months. Conclusion: The liver-targeted BSA liposomes were successfully prepared and have good stability and high drug encapsulation efficiency, which can be used as a new model for liver-targeted drug delivery of protein drugs.