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为了研究黑土长期定位试验原状土整体搬迁对土壤细菌群落结构多样性的影响,采用PCR-DGGE及其特异性条带克隆测序的方法,对同为小麦茬的搬迁前2010年和搬迁后2013年土壤细菌群落结构进行研究。结果表明:细菌DGGE图谱中搬迁前后两个土层不同处理所有样品之间具有相似性,进一步对细菌DGGE图谱进行聚类分析可得,细菌群落结构在两个土层各处理中搬迁前后变化不大。再对细菌DGGE图谱主成分分析结果可得,在0~20 cm土层细菌各处理的分布虽然搬迁前和搬迁后产生分异,但在搬迁前后两年中处理间的分布规律相似,在20~40 cm土层搬迁前和搬迁后细菌的MNPK和NPK处理分布规律发生了变化。研究说明,搬迁前和搬迁后土壤中细菌群落结构受到耕作方式、植被类型、土壤肥力等的影响远大于搬迁的扰动影响影响。
In order to study the effect of long-term positioning experiment of intact native soil on the diversity of soil bacterial community structure, PCR-DGGE and its specific banding cloning sequencing method were used to study the effects of long- Soil bacterial community structure was studied. The results showed that there was a similarity between all the samples of two soil layers before and after the removal of DGGE, and further analysis of the bacterial DGGE patterns by cluster analysis showed that bacterial community structure did not change before and after the relocation of the two soil layers Big. The results of principal component analysis of the bacterial DGGE patterns showed that although the distribution of bacteria in the 0 ~ 20 cm soil layer was different before and after the relocation, the distribution of bacteria in the two years before and after the relocation was similar, The changes of distribution of MNPK and NPK in ~ 40 cm soil layer before and after the removal of bacteria were changed. The research shows that the bacterial community structure in the soil before and after the relocation is far more affected by the tillage pattern, vegetation type and soil fertility than the disturbance effect of the relocation.