论文部分内容阅读
第一代B群脑膜炎球菌外膜蛋白(OMP)菌苗对预防细菌性脑膜炎已有一定的效果,由于Ⅰ类OMP异质性有限,故应研制多价Ⅰ类OMP菌苗.作者选用两株B群脑膜炎球菌Ⅰ类OMP基因工程菌PL16215和PL10124制备菌苗,每株工程菌分别表达三种不同的Ⅰ类OMP,两株菌共表达P1.7,16、P1.5,2、P1.19,15和P1.5,10、P1.12,13、P1.7,4六个亚型.用脱氧胆酸盐(DOC)从工程菌中抽提外膜囊(OMV),与磷酸铝佐剂混合制成菌苗.作者用免疫化学和生化技术对OMV菌苗的成分进行鉴定.结果显示,OMV大小约
The first generation of meningococcal outer membrane protein (OMP) vaccine to prevent bacterial meningitis has had some effect, due to the limited heterogeneity of Class I OMP, it should develop multivalent Class I OMP vaccine. Two strains of meningococcal serogroup I OMP gene engineering bacteria PL16215 and PL10124 were used to prepare the bacterins. Three different class I OMPs were expressed in each strain of engineering bacteria, and the two strains co-expressed P1.7,16, P1.5,2 , P1.19,15 and P1.5,10, P1.12,13, P1.7,4 six subtype.Using the deoxycholate (DOC) from the engineering bacteria extract outer capsule (OMV) And aluminum phosphate adjuvant to make bacterin.The authors used immunochemical and biochemical techniques to identify the components of OMV bacterin.The results showed that the size of OMV