苹果、李和樱桃等几种果树,已通过离体培养技术进行了无性繁殖。某些柑桔砧木虽可通过珠心实生苗进行无性繁殖,但由于新引进的砧木必需好几年才能产生足够的种子,所以用非珠心的营养组织来进行微型繁殖大概是较理想的。某些砧木无性系虽然具有很多优点,但却可能不产生珠心实生苗,或者可能果实无核或种子极少;而接穗品种嫁接在用枝条无性繁殖而成的砧木上,其结果期比嫁接在用种子珠心无性繁殖而成的砧木上的早。因此我们进行了下述研究,以确定用茎尖组织培养的方法 Several fruit trees, such as apple, plum and cherry, have been asexually propagated through in vitro culture techniques. Although some citrus rootstocks can be vegetatively propagated through seedling kernels, it is probably desirable to micropropagrate with non-nodding vegetative tissue since the newly introduced rootstocks take several years to produce sufficient seeds. Although some rootstock clones have many advantages, some rootstock clones may not produce seedling seedlings, or may have no fruit or seedless seeds; while the scion varieties are grafted onto rootstocks asexually propagated with branches, the result period is longer than that of grafting Asexual propagation of seed beads in the seed early. Therefore, we conducted the following study to determine the method of tissue culture with shoot tips