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用Nafion-甲基紫精修饰电极为基底,以牛血清白蛋白戊二醛为交联剂,将丙铜酸氧化酶固定在电极上,制成丙酮酸氧化酶生物传感器.用这种生物传感器测定人体血清中谷丙转氨酶(GPT)的活性,共存的尿酸、抗坏血酸等电活性物质不干扰测定.测定GPT的活性范围为0～110U/L,响应时间为50s.该传感器具有灵敏度高、抗干扰能力强和响应快等特点.
Using Nafion-methyl viologen modified electrode as substrate and bovine serum albumin glutaraldehyde as cross-linking agent, copper pyruvate oxidase was immobilized on the electrode to prepare pyruvate oxidase biosensor. With this biosensor The activity of alanine aminotransferase (GPT) in human serum and the coexistence of uric acid, ascorbic acid and other electroactive substances did not interfere with the determination.The activity of GPT ranged from 0 to 110 U / L and the response time was 50 s.The sensor has high sensitivity and anti-interference Strong ability and response characteristics.