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以前的研究发现：ＨＢＶＸ蛋白能与肿瘤抑制基因ｐ５３蛋白结合，在原发性肝癌的发生中具有重要意义。为进一步探讨二者之间的关系，经过计算机比较分析发现，在ＨＢＶ基因组中存在与经典的ｐ５３ＤＮＡ－蛋白质结合位点类似的序列（ＴＧＣＣＴ），用含有此序列的ＨＢＶ基因片段作探针，与肝癌细胞核蛋白作用，通过凝胶电泳迁移率移位实验、凝胶电泳迁移率超移位实验和原位紫外线交联实验，确定其ＨＢＶＤＮＡ与ｐ５３蛋白的特异性结合；进一步用ｐ５３与ＨＢＶＤＮＡ共转染和定量ＰＣＲ分析，探讨二者结合对ＨＢＶＤＮＡ复制的影响和意义。结果显示，ＨＢＶ基因组中存在能与ｐ５３蛋白特异性结合的序列，使细胞内ｐ５３蛋白积聚，并反式激活，增强ＨＢＶＤＮＡ的复制。这一结果对进一步揭示ＨＢＶ感染与原发性肝癌发生的关系，具有非常重要的意义
Previous studies have found that: HBVX protein with tumor suppressor p53 protein binding, in the occurrence of primary liver cancer is of great significance. To further explore the relationship between the two, we found by computer analysis that there is a similar sequence (TGCCT) in the HBV genome to the classical p53 DNA-protein binding site. Using the HBV gene fragment containing this sequence as a probe, The nuclear protein of hepatocellular carcinoma cells was detected by gel electrophoretic mobility shift assay, gel electrophoresis mobility shift assay and in-situ UV cross-linking test to determine the specific binding of HBVDNA to p53 protein. The co-transfection of p53 and HBVDNA Staining and quantitative PCR analysis to explore the combination of the two on HBVDNA replication and its significance. The results showed that there is a sequence in the HBV genome that can specifically bind to p53 protein, which causes the accumulation of p53 protein in the cell and transactivation to enhance the replication of HBVDNA. This result is very important to further reveal the relationship between HBV infection and the occurrence of primary liver cancer