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目的探讨红薯叶黄酮对糖尿病模型大鼠肾的保护作用及其可能机制。方法采用链脲佐菌素(STZ65mg/kg)腹腔注射复制糖尿病大鼠模型,并以不同剂量的红薯叶黄酮灌胃,给药8周后检测相关指标,光镜观察肾组织形态学变化。采用原位末端标记法检测大鼠肾细胞凋亡指数,以免疫组织化学法检测Bcl-2/Bax的表达。结果红薯叶黄酮可降低糖尿病大鼠血糖、血尿素氮、尿微量白蛋白、血肌酐、肾重/体重比值和肌酐清除率水平(P<0.01),呈剂量效应关系。与模型组相比,高剂量红薯叶黄酮组大鼠肾小球结构病理改变明显减轻,且凋亡细胞数减少、Bax表达减弱、Bcl-2表达增强、Bax/Bcl-2降低(P<0.01)。结论红薯叶黄酮可能通过调节凋亡相关基因bax和bcl-2表达而减少肾细胞凋亡,从而发挥保护肾脏作用。
Objective To investigate the protective effect of sweet potato leaf flavonoids on the kidney of diabetic rats and its possible mechanism. Methods Diabetic rats were injected intraperitoneally with streptozotocin (STZ65mg / kg), and were given different doses of flavonoids in sweet potato leaves. After 8 weeks of administration, the relevant indexes were detected. Morphological changes of kidney were observed with light microscope. Rat renal cell apoptosis index was detected by in situ end labeling, and the expression of Bcl-2 / Bax was detected by immunohistochemistry. Results Flavone of sweet potato leaves could reduce the level of blood glucose, blood urea nitrogen, urinary albumin, serum creatinine, renal weight / body weight ratio and creatinine clearance rate (P <0.01) in diabetic rats. Compared with the model group, the pathological changes of glomerulus in high-dose sweet potato leaf flavonoids group were significantly reduced, and the number of apoptotic cells was decreased, the expression of Bax was decreased, the expression of Bcl-2 was increased, Bax / Bcl-2 was decreased ). Conclusions Flavonoids from sweet potato can reduce renal cell apoptosis by regulating the expression of bax and bcl-2, and thus play a role in protecting kidney.