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目的:分离江苏省2011年流行性腮腺炎病毒(Mumps Virus,MuV),并分析其基因特征。方法:对江苏省2011年腮腺炎咽拭子进行病毒分离,对MuV分离株针对SH基因进行PCR扩增,并对该PCR产物进行序列测定,结合从基因库下载的中国MuV毒株序列以及世界卫生组织(WHO)MuV基因型参考株一起进行分子流行病学研究。结果:通过比较核苷酸和氨基酸同源性和构建亲缘关系树发现,江苏省2011年MuV分离株属F基因型,但序列相互间存在差异。此外,通过分析江苏省2011年MuV分离株的遗传距离发现,MuV毒株序列同源性和亲缘关系树分析结果一致。结论:江苏省2011年流行的MuV同属F基因型,是由F基因型MuV的多个传播链引起的。并且2011年的MuV毒株间存在序列差异,说明在江苏省流行的MuV发生了一定程度的变异。
Objective: To isolate Mumps Virus (MuV) from 2011 in Jiangsu Province and analyze its genetic characteristics. Methods: Throat swab from mumps was isolated from Jiangsu province in 2011, and the SH gene was amplified by PCR from MuV isolate. The sequence of the PCR product was sequenced. Combined with the sequence of Chinese MuV strain downloaded from the GenBank, WHO (WHO) MuV genotype reference strains together molecular epidemiological studies. Results: By comparing nucleotide and amino acid homology and constructing phylogenetic tree, it was found that MuV isolates in Jiangsu Province were F genotype in 2011, but the sequences were different from each other. In addition, by analyzing the genetic distance of MuV isolates in Jiangsu Province in 2011, it was found that the sequence homology and phylogenetic tree of MuV strains were consistent. Conclusion: The MuV genotypes prevailing in Jiangsu province in 2011 are caused by multiple transmission chains of F genotype MuV. And there was a sequence difference between the MuV strains in 2011, indicating some degree of variation in the MuV epidemic in Jiangsu Province.