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将枯草芽孢杆菌B－903菌株液体培养72h后，将培养滤液高温灭菌，以不同比例将培养滤液加入镰刀菌孢子悬浮液中，置于扫描电镜和光学显微镜下定时观察。在处理12h后，镰刀菌孢子芽管顶端、菌丝末端及菌丝中央的多处细胞均可发生畸形的球状结构，这种畸变结构随处理时间延长而增加，致使菌丝变成捻珠状。处理36h后，畸变球形细胞及菌丝纷纷断裂离解，细胞内含物渗出，致使大部分细胞成为空泡。到处理48h后，镜下几乎无完整菌丝，均成单个的球状细胞空泡，这些空泡最终胞壁崩裂，不复存在。笔者认为正是枯草芽孢杆菌在生活过程中分泌到体外的抗高温代谢物对镰刀菌孢子和菌丝细胞的这种畸变作用造成拖子和菌丝失活，侵染能力丧失。
Bacillus subtilis B-903 strain liquid culture 72h, the culture filtrate was sterilized at different temperatures, the culture filtrate was added to the Fusarium spore suspension, placed in scanning electron microscopy and optical microscopy under regular observation. After 12 h of treatment, the deformed spherical structures were observed at the top of sporophyll of Fusarium spores, at the ends of mycelium and in the middle of mycelium. This distorted structure increased with the prolongation of treatment time, . 36h after treatment, the deformed spherical cells and mycelium have broken off dissociation, cell exudation, resulting in the majority of cells become vacuolar. After treatment for 48h, there were almost no hyphal hyphae in the microscope, all of them became single globular cell cavities, which finally cracked and disappeared. I believe it is Bacillus subtilis in the process of life excreted in vitro anti-high temperature metabolites of Fusarium spores and hyphal cells of this distortion caused by drag and mycelium inactivation, loss of infectivity.