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[目的]探讨活性氧(ROS)在900 MHz微波辐射诱导γ射线致小鼠骨髓基质细胞DNA损伤的适应性反应中的作用。[方法]给予小鼠骨髓基质细胞120μW/cm~2,900 MHz微波照射,4 h/d,连续5 d,微波照射结束4 h后给予1.5 Gyγ射线照射,照射结束后立即采用碱性单细胞凝胶电泳试验和γ-H2AX焦点形成试验检测DNA损伤,观察微波辐射诱导的适应性反应;采用500 nmol/L褪黑素(ROS抑制剂)在微波照射前4 h处理细胞,流式细胞仪检测ROS水平的变化,观察适应性反应的变化情况。[结果]微波照射后细胞内ROS水平(2.10)较对照组(1.16)升高(P<0.01)。与单独给予γ射线照射的彗星尾长、尾矩和γ-H2AX焦点形成数(分别为21.62μm、5.68、19.73)相比,预先给予微波照射可明显减轻随后γ射线照射造成的DNA损伤,彗星尾长、尾矩和γ-H2AX焦点形成数(分别为8.57μm、2.58、5.28)明显减少(P<0.001),诱导出适应性反应。褪黑素预处理细胞后,微波诱导产生的ROS下降为1.53(P<0.01),微波拮抗γ射线照射造成的DNA损伤程度也随之下降,彗星尾长、尾矩和γ-H2AX焦点形成数(分别为17.90μm、4.10、20.8)明显升高(P<0.001),适应性反应受到抑制。[结论]ROS可能在900 MHz微波辐射诱导细胞适应性反应中发挥作用。
[Objective] To investigate the role of reactive oxygen species (ROS) in adaptive response to DNA damage induced by γ-rays induced by 900 MHz microwave in bone marrow stromal cells of mice. [Method] The mouse bone marrow stromal cells were irradiated by microwave at 120μW / cm ~ 2,900 MHz for 4 h / d for 5 d. After 4 h of microwave irradiation, 1.5 Gy γ - ray irradiation was given. After the irradiation, alkaline single cell gel Electrophoresis and γ-H2AX Focal Adhesion Assay were used to detect DNA damage and observe the adaptive response induced by microwave irradiation. Cells were treated with 500 nmol / L melatonin (ROS inhibitor) for 4 h before microwave irradiation. ROS was detected by flow cytometry Level changes observed changes in adaptive response. [Results] The level of intracellular ROS (2.10) after microwave irradiation was higher than that of control group (1.16) (P <0.01). Compared with the tail length, tail moment and focal spot number of γ-H2AX (21.62μm, 5.68,19.73, respectively), the pretreatment with microwave irradiation could obviously reduce the DNA damage induced by γ-ray irradiation, and the comet Tail length, tail moment and γ-H2AX focal spot formation number (8.57μm, 2.58,5.28, respectively) were significantly decreased (P <0.001), induced adaptive response. After pretreated with melatonin, ROS decreased by 1.53 (P <0.01). The DNA damage induced by microwave irradiation was also decreased, and the tail length, tail moment and γ-H2AX focal spot formation number (17.90μm, 4.10,20.8 respectively) was significantly higher (P <0.001), adaptive response was inhibited. [Conclusion] ROS may play a role in cell adaptive response induced by 900 MHz microwave radiation.