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以芒果品种贵妃、金煌及其98株杂交F_1代为材料,利用SRAP标记构建了芒果的遗传图谱,并对杂交后代的遗传多样性进行分析。从266对SRAP引物中筛选了35对多态性引物,扩增出200条多态性条带,多态性条带比率为43.18%。UPGMA聚类分析表明,材料间的遗传相似系数为0.52~0.92,有8份材料表现出较强的变异。用JoinMap4.0作图,构建了一张总长801.6 cM的芒果遗传图谱,包含20个连锁群,113个标记位点,标记间的平均距离为9.32 cM。这是首张芒果SRAP标记的遗传图谱,可为芒果高密度遗传图谱的构建提供参考。
Genetic linkage maps of mango were constructed with SRAP markers, and the genetic diversity of hybrid progeny was analyzed using the F1 hybrids of Magnolia, Jinhuang and their 98 hybrids. Thirty-six polymorphic primers were screened from 266 SRAP primers and 200 polymorphic bands were amplified. The percentage of polymorphic bands was 43.18%. UPGMA cluster analysis showed that the genetic similarity coefficient between materials was 0.52 ~ 0.92, and 8 materials showed strong variation. With JoinMap4.0, we constructed a genetic map of mango with length of 801.6 cM, including 20 linkage groups and 113 marker sites with an average distance of 9.32 cM. This is the first mango SRAP marker genetic map, which can provide a reference for the construction of mango high-density genetic map.