玉米赤霉烯醇具有雌激素的作用,可使家禽和家畜的雌激素水平提高,同时对雄性动物也有一定的影响,在急性中毒的条件下,使动物突然死亡。本文以液相色谱-串联质谱法测定饲料中的玉米赤霉烯醇,经试验:玉米赤霉烯醇在5~500ng/mL的浓度范围内呈现良好的线性关系,相关系数均≥0.999;检测限能达到10μg/kg,定量限能达到20μg/kg;回收率均在60%~110%范围内;批内、批间相对标准偏差均≤15%。 Zearalenol, which acts as an estrogen, increases estrogen levels in poultry and livestock and at the same time exerts some effects on males, causing sudden death in animals under acute poisoning conditions. In this paper, the determination of zearalenol in feed by liquid chromatography-tandem mass spectrometry showed that the calibration curve of zearalenol in the range of 5 ~ 500ng / mL showed a good linear relationship with a correlation coefficient of ≥0.999. The limit of quantification reached 10μg / kg and the limit of quantitation reached 20μg / kg. The recoveries ranged from 60% to 110%. The relative standard deviations (RSDs) within and between batches were both ≤15%.