论文部分内容阅读
目的:探讨辐射诱发人支气管上皮细胞癌变株和肺癌组织中DNA依赖蛋白激酶(DNA dependent protein kinase, DNA-PK)的激酶活性或表达变化。方法:用Western blot和p53蛋白为特异性底物的磷酸化反应分别检测癌变细胞中DNA-PKcs表达和激酶活性,用免疫组化结合病理图像定量分析技术检测肺癌组织和癌旁组织中DNA-PKcs蛋白的表达情况。结果:a粒子辐射诱发的人支气管上皮细胞癌变株BERP35T-1的DNA-PKcs表达水平比亲本细胞BEP2D提高30%,激酶活性显著提高。所检测的14例非小细胞肺癌组织中DNA-PKcs蛋白表达水平普遍高于相对应的癌旁组织,两组之间有显著性差异(P < 0.05)。结论:非小细胞肺癌组织中DNA-PKcs表达增加,可能成为肺癌的一个生物标记物。
Objective: To investigate the kinase activity or the expression of DNA-dependent protein kinase (DNA-PK) in radiation-induced human bronchial epithelial cell carcinoma and lung cancer. Methods: DNA-PKcs expression and kinase activity in cancerous cells were detected by Western blot and p53 protein-specific substrate phosphorylation respectively. Immunohistochemistry and pathological image quantitative analysis were used to detect the DNA- PKcs protein expression. Results: The DNA-PKcs expression level of BERP35T-1 in a human bronchial epithelial cell line induced by a particle irradiation was increased by 30% and the kinase activity was significantly increased compared with the parental cell line BEP2D. The expression level of DNA-PKcs in 14 non-small cell lung cancer tissues was generally higher than that in corresponding paracancerous tissues, with significant difference between the two groups (P <0.05). Conclusion: The increased expression of DNA-PKcs in non-small cell lung cancer may be a biomarker of lung cancer.