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从安徽芜湖、巢县、肥东、合肥四地区感染白叶枯病的稻叶、病谷及病土中分离出12株噬菌体,进行生物学性状测定。结果表明:各株噬菌体所测性状相似,属同一类型。以P_ch为代表,在平板上培养24小时,形成溶菌斑的直径为4—5毫米;热钝化温度为63℃;除白叶枯细菌外,对其它多种供试植物病原细菌均无侵染力;潜育期为45分钟,上升期20分钟,平均裂解量47倍,易为三价铁盐钝化。分别以P_ch和OP_3噬菌体作为免疫抗原,制备噬菌体抗血清。用抗血清除去游离噬菌体,平板培养测定吸附态噬菌体形成的溶菌斑数,来确定白叶枯细菌的活菌量,可以使10~3个/ml浓度以上的活细菌被检测出来并进行定量。
Twelve phages were isolated from rice leaves, diseased valleys and diseased soils infected with bacterial blight in Wuhu, Chao County, Feidong, Hefei, Anhui Province, and the biological characteristics were determined. The results showed that the tested strains of each strain were similar and belong to the same type. P_ch as the representative, on the plate for 24 hours to form a plaque diameter of 4-5 mm; heat passivation temperature of 63 ° C; in addition to bacterial leaf beetles, the other tested plant pathogenic bacteria have no invasion Dyeing; incubation period of 45 minutes, 20 minutes rise, the average amount of 47 times the amount of cracking, easily ferric salt passivation. Phage antiserum was prepared by using P_ch and OP_3 phage as immune antigens respectively. Free phage is removed with antiserum and the number of bacterioplast formed by the adsorbed bacteriophages is determined in a plate culture to determine the viable cell count of Bacillus subtilis so that viable bacteria at a concentration of 10 to 3 / ml can be detected and quantified.