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目的建立人血浆3-氯酪氨酸(3-CT)浓度的定量分析方法。方法用三氯乙酸沉淀血浆蛋白后,HPLC-ECD定量检测。结果3-氯酪氨酸血浆标准曲线的线性范围为0.062 5~2.000μmol.L-1,r>0.996 6,回收率>71.8%。日内和日间的精密度(RSD%)分别<8.8%和11.1%。3-氯酪氨酸的保留时间为(16.57±0.30)min,总分析时间32 min。测得健康人和尿毒症患者(各6例)血浆3-CT浓度分别为(0.16±0.13)和(2.47±0.73)μmol.L-1(P<0.01)。结论本法所需样本量低,预处理步骤简便,适用于临床进行氧化应激、治疗、预后、诊断等研究工作之需。
Objective To establish a quantitative method for the determination of 3-chloro-tyrosine (3-CT) in human plasma. Methods The plasma protein was precipitated with trichloroacetic acid and quantified by HPLC-ECD. Results The linear range of 3-chloro-tyrosine plasma standard curve was 0.062 5 ~ 2.000 μmol.L-1, r> 0.996 6. The recovery rate was> 71.8%. Intra-day and inter-day precision (RSD%) were <8.8% and 11.1%, respectively. The retention time of 3-chloro-tyrosine was (16.57 ± 0.30) min, the total analysis time was 32 min. Plasma concentrations of 3-CT were measured in healthy and uremic patients (6 in each) as (0.16 ± 0.13) and (2.47 ± 0.73) μmol.L-1, respectively (P <0.01). Conclusion This method requires low sample size and simple pretreatment steps and is suitable for the clinical research of oxidative stress, treatment, prognosis and diagnosis.