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目的探讨运用不同填充剂与透明方式制作小鼠回肠血管透明标本的方法,评价其优缺点。方法运用简易毛细管黏滞计测定填充剂的黏滞系数。8只小鼠被分为4组。采用改良灌流方法清洗血管,分别注入不同的填充剂,用不同的透明方法处理,比较肠组织和透明剂的折光指数。结果硝化纤维素填充剂的黏滞系数、填充效果均优于其他组;20℃下,肠组织与透明剂折光指数差值分别为甘油法:2.2×10-3n D,透明-固定液法:65.8×10-3n D。结论以硝化纤维素填充剂填充血管,使用透明-固定液法处理后,得到的标本效果较好。
Objective To explore the method of using different fillers and transparent method to make transparent samples of ileal vasculature of mice to evaluate the advantages and disadvantages. Methods The viscous coefficient of filler was measured by simple capillary viscometer. Eight mice were divided into four groups. Blood vessels were cleaned by modified perfusion method, and different fillers were injected respectively. Different transparent methods were used to compare the refractive index of intestinal tissue and the transparent agent. Results The viscosity coefficient and filling effect of nitrocellulose filler were better than those of other groups. The difference of refractive index of intestinal tissue and transparent agent at 20 ℃ were glycerol method: 2.2 × 10-3n D, transparent-fixative method: 65.8 × 10 -3n D. Conclusion The blood vessels filled with nitrocellulose filler, the use of transparent - fixed solution method, the specimens obtained better.