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目的:研究苦参碱和甘草酸在小肠各段和不同剂量下的吸收特征,探索两种成分联用时的吸收变化。方法:采用大鼠单向肠灌流模型,利用HPLC法测定灌流液中苦参碱和甘草酸的含量变化,计算其吸收速率参数(K_a)和有效渗透系数(P_(eff))。结果:苦参碱在小肠各段均有吸收且空肠段吸收最好与十二脂肠和回肠的吸收参数差异有统计学意义(P<0.05);不同质量浓度下吸收差异有统计学意义(P<0.05)。甘草酸在小肠吸收较差,不同肠段和质量浓度下的吸收差异无统计学意义(P>0.05)。两种物质联用时,苦参碱和甘草酸的吸收与单用时相比差异有统计学意义(P<0.05)。结论:苦参碱的吸收呈剂量相关性,随质量浓度的增加而增加。甘草酸的吸收不随质量浓度变化而改变,吸收方式可能为被动转运。两种物质联用时可以提高苦参碱的体内吸收和甘草酸的肠内浓度。
OBJECTIVE: To study the absorption characteristics of matrine and glycyrrhizic acid in the small intestine and in different doses, and to explore the absorption changes of the two components in combination. Methods: The rat model of unilateral intestinal perfusion was established. The contents of matrine and glycyrrhizic acid in the perfusate were determined by HPLC. The absorption rate (K_a) and effective permeation coefficient (P_ (eff)) were calculated. Results: Matrine was absorbed in all sections of the small intestine and the absorption was best in the jejunal segment and the absorption parameters of the 12-intestine and the ileum were statistically different (P <0.05). The absorption of matrine at different concentrations was statistically significant P <0.05). Glycyrrhizic acid absorption in the small intestine poor, different intestinal segments and the absorption of mass concentration was no significant difference (P> 0.05). The combination of two substances, matrine and glycyrrhizic acid absorption compared with single-use difference was statistically significant (P <0.05). Conclusion: Matrine absorption in a dose-dependent manner, with the increase of mass concentration. The absorption of glycyrrhizin does not change with the change of mass concentration, the absorption mode may be passive transport. The combination of two substances can increase matrine in vivo absorption and glycyrrhizic intestinal concentration.