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为了探讨非侵蚀性葡萄胎（ＨＭ）转化为侵蚀性葡萄胎（ＩＨＭ）的细胞生物学机制，本研究采用免疫组织化学法观察了ＮＧＦ和ＴＧＦ－β１在ＨＭ和ＩＨＭ中的分布。结果显示：在ＨＭ绒毛中，合体滋养细胞（ＳＴ）和新生基质细胞ＮＧＦ和ＴＧＦ－β１呈强阳性和阳性；老化基质细胞和细胞滋养细胞（ＣＴ）呈阴性；在ＨＭ的中间型滋养细胞，ＮＧＦ呈强阳性着色，ＴＧＦ－β１阴性；在ＨＭ绒毛干中，ＳＴ的ＮＧＦ呈强阳性，ＮＧＦ和ＴＧＦ－β１呈弱阳性；ＣＴ的ＮＧＦ呈弱阳性，ＮＧＦ和ＴＧＦ－β１为阴性；在ＨＭ的绒毛干近滋养层基质细胞，ＮＧＦ呈弱阳性，ＮＧＦ和ＴＧＦ－β１呈阳性；在远滋养层基质细胞，免疫组化染色皆为阴性。在ＩＨＭ的绒毛ＳＴ和ＣＴ免疫组化染色皆为阴性，在绒毛新生基质细胞，免疫组化染色呈阳性；在绒毛老化基质细胞，皆为阴性
In order to investigate the cellular mechanism of non-invasive hydatidiform mole (HM) conversion to invasive hydatidiform mole (IHM), immunohistochemistry was used to observe the distribution of NGF and TGF-β1 in HM and IHM. The results showed that there was a strong positive and positive expression of NGF and TGF-β1 in syncytiotrophoblasts (ST) and neonatal stromal cells in HM villi, and aging stromal cells and cytotrophoblasts (CT) were negative. In intermediate HM trophoblastic cells, NGF and TGF-β1 were weakly positive in CTL, while NGF and TGF-β1 were negative in CT; Of the hairy trophoblast stromal cells, NGF was weakly positive, NGF and TGF-β1 positive; in the far trophoblast stromal cells, immunohistochemical staining were negative. Immunohistochemical staining of both ST and CT in the chorionic villi of IHM was negative, and in the villous neonatal stromal cells, immunohistochemical staining was positive. In the villous aging stromal cells, all were negative