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从五种不同土壤类型中分离获得土著大豆根瘤菌512株.固氮活性以淡黑钙土分离的根瘤菌群为最高,依次为黑土、草甸黑土、白浆土和轻碱土.在黑土、草甸黑土和白浆土中以477—28和477—694根瘤菌血清群(国内2028血清群)为主,淡黑钙土中以35血清群(USDA 124血清群)为主.轻碱土中以快生型根瘤菌为主,黑土和草甸黑土中也存在有快生型但和白浆土、淡黑钙土一样以慢生型为主.土著大豆根瘤菌中有5.25%菌株具有吸氢作用.大豆根上的结瘤随大豆生长而不断增加,一直到结荚鼓粒后期(R_6).根瘤数目的增加主要是侧根上根瘤数目的增加.
512 strains of indigenous soybean rhizobia were isolated from five different soil types.The root nodulation bacteria with nitrogen fixation activity separated by light chernozem soil were the highest, followed by black soil, meadow black soil, white soil soil and light alkaline soil, Melanogaster strains 477-28 and 477-694 serogroups (domestic 2028 serogroups) are dominant in meadow black soil and albic soil, while 35 serogroups (USDA 124 serogroups) Fast-growing rhizobia, black soil and meadow black soil also exist in the fast-growing type, but the same as the white earth, light chernozem to slow-born type mainly indigenous indigenous Rhizobium strains 5.25% of the strains have hydrogen absorption Effect.The nodulation of soybean root increased with the growth of soybean until the late stage of pod formation (R_6) .The increase of nodule number was mainly the increase of nodule number on lateral root.