以光敏核不育水稻农垦58S为材料,在杭州中国水稻研究所人工气候箱(Koitotron S-153W)群中进行光周期处理。自秧苗6叶期开始,先经10h/d的短日照处理10d,到幼穗开始分化,然后分别在13、14h/d的光周期条件下发育。通过透射电镜观察探索光周期在决定花粉育性中的作用。结果发现,在13h/d光周期条件下,花粉发育正常:而在14h/d的光周期条件理、花粉在单核晚期败育、表现在核糖体呈聚合状态,内质网、质体、线粒体等细胞器逐渐解体、缺乏淀粉积累,吞噬泡增多,细胞质稀薄,最终变成不育。另一方面,绒毡层细胞保持完整状态,无解体迹象。 Photoperiod-sensitive genic male-sterile rice Nongken 58S was used as photoperiod in the Koitotron S-153W cluster at the Rice Research Institute of Hangzhou, China. Since the 6-leaf stage of seedling, the young panicles began to differentiate after short-day treatment of 10h / d for 10 days and then developed under the photoperiod of 13 and 14h / d respectively. The role of photoperiod in determining pollen fertility was explored by transmission electron microscopy. The results showed that the pollen development was normal under the 13h / d photoperiod. In the photoperiod of 14h / d, the pollen was aborted at the late stage of mononuclear stage, which showed that the ribosomes were aggregated, the endoplasmic reticulum, plastid, Organelles and other organelles gradually disintegrated, the lack of starch accumulation, phagocytic increase, thin cytoplasm, and eventually become sterile. On the other hand, tapetum cells remained intact with no evidence of disintegration.