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气道炎症,过敏反应,杯状细胞数目增多和粘液产生过度是哮喘的特征。几个实验室观察到应用卵白蛋白(OVA)刺激致敏的小鼠引起与人过敏性哮喘相似的肺病理学变化。最近发现,白介素(IL)-13作为中心介质参与这一过程。由于健康小鼠有少量粘液细胞,这些细胞数目的增多,可能反映出粘液基因表达的诱导。本研究的目的是确定在应用OVA刺激或单独应用IL-13处理致敏小鼠中杯状细胞化生(GCV)与粘液素基因表达诱导之间的关系。用100μg(溶于生理盐水)OVA通过腹膜内注射(0,4,7,11和14天)和鼻内滴注(14天)致敏BALB/C小鼠,然后经用鼻内滴注(25,26
Airway inflammation, anaphylaxis, an increase in goblet cells, and excessive mucus production are hallmarks of asthma. Several laboratories have observed that administration of ovalbumin (OVA) -stimulated mice elicits similar lung pathology as human allergic asthma. It has recently been discovered that interleukin (IL) -13 is involved as a central mediator in this process. Since healthy mice have a few myxoid cells, the increased number of these cells may reflect the induction of mucus gene expression. The purpose of this study was to determine the relationship between goblet metaplasia (GCV) and induction of mucin gene expression in sensitized mice treated with OVA stimulation or with IL-13 alone. BALB / C mice were sensitized with 100 μg OVA in saline (0, 4, 7, 11 and 14 days) and intranasal instillation (14 days), followed by intranasal instillation 25,26