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目的:比较黄芩炒炭前后挥发性成分的含量变化,为阐明其炮制机制提供参考。方法:利用水蒸气蒸馏法提取黄芩中挥发油,利用GC-MS分析黄芩炮制品中挥发油成分的组成及含量变化,DB-5MS气相色谱柱(0.25 mm×30 m,0.25μm),载气为氦气,电子轰击电离源,离子源温度280℃,质谱接口温度280℃,电子能量70 e V。全离子扫描,质量扫描范围m/z 33~350,流速40 m L·min~(-1),分流比5∶1,采集延时2 min,图谱采集时间50.0 min。通过检索比对NIST-05标准质谱图谱库,鉴定样品挥发油中化学成分。结果:生黄芩、黄芩炭中挥发油提取量分别为1.3,0.8μL·g~(-1),GC-MS共鉴定了50种挥发油成分,其中生黄芩25种,黄芩炭31种。根据峰面积归一化法分析,鉴定的成分分别占生黄芩、黄芩炭饮片挥发油总量的100.00%,99.93%。结论:黄芩炒炭后挥发性成分含量有所降低,挥发性成分组成有所改变,但炒炭后挥发性成分尚存,可为阐释黄芩“炒炭存性”的炮制机制提供科学依据。
OBJECTIVE: To compare the changes of the volatile components before and after the scutellaria baicalensis Georgi, providing a reference for elucidating its processing mechanism. Methods: Volatile oil from Radix Scutellariae was extracted by steam distillation. The composition and content of volatile oil in Radix Scutellariae were analyzed by GC-MS. DB-5MS gas chromatographic column (0.25 mm × 30 m, 0.25 μm) Gas and electron bombardment ionization source, ion source temperature 280 ℃, mass spectrometry interface temperature 280 ℃, electron energy 70 eV. All ion scan, mass scan range m / z 33 ~ 350, flow rate 40 m L · min ~ (-1), split ratio 5: 1, collection delay 2 min, spectrum acquisition time 50.0 min. The chemical composition of volatile oil was identified by searching and comparing the NIST-05 standard spectrum library. Results: The volatile oil contents of Scutellaria baicalensis and Scutellaria baicalensis were 1.3 and 0.8 μL · g -1, respectively. A total of 50 volatile oil components were identified by GC-MS, including 25 species of Scutellaria baicalensis and 31 species of Scutellaria baicalensis. According to the peak area normalization method, the identified components accounted for 100.00% and 99.93% of the total volatile oils of Scutellaria baicalensis and Scutellaria baicalensis. Conclusion: The content of volatile components of Scutellaria baicalensis Georgi decreased, the composition of volatile components changed, but the volatile components of Scutellaria baicalensis remaining after storage could provide a scientific basis for explaining the processing mechanism of Scutellaria baicalensis. .