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目的研究Toll样受体7激动剂(Gardiquimod)对人γδT细胞杀伤肿瘤作用的影响。方法以异戊烯焦磷酸法扩增人外周血γδT细胞。用不同浓度的Gardiquimod处理γδT细胞和肺癌细胞A549,MTT法检测细胞增殖情况。LDH法检测Gardiquimod处理后γδT细胞对A549的杀伤活性;流式细胞术检测处理前后γδT细胞上CD107a、穿孔素和颗粒酶的表达情况。结果 Gardiquimod在5.0~0.31μg/ml浓度可明显促进γδT细胞增殖,同时抑制A549增殖。Gardiquimod处理γδT细胞后,杀伤A549能力明显增强,且CD107a、颗粒酶及穿孔素的表达显著升高,与对照组相比,有统计学差异(P<0.05)。结论 Gardiquimod通过增高γδT细胞上的CD107a、颗粒酶及穿孔素的表达来增强其杀伤肿瘤作用。
Objective To investigate the effect of Toll-like receptor 7 agonist (Gardiquimod) on the cytotoxicity of human γδT cells. Methods The isopentenyl pyrophosphate method was used to amplify human peripheral blood γδT cells. ΓδT cells and lung cancer cells A549 were treated with different concentrations of Gardiquimod, and the cell proliferation was detected by MTT assay. The cytotoxicity of γδT cells to A549 by Gardiquimod was detected by LDH assay. The expression of CD107a, perforin and granzyme on γδT cells was detected by flow cytometry. Results Gardiquimod at a concentration of 5.0 ~ 0.31μg / ml could significantly promote the proliferation of γδT cells and inhibit the proliferation of A549 cells. Gardiquimod treatment of γδT cells, the ability to kill A549 significantly increased, and CD107a, granzyme and perforin expression was significantly increased, compared with the control group, there was a statistically significant difference (P <0.05). Conclusion Gardiquimod enhances its anti-tumor effect by increasing the expression of CD107a, granzyme and perforin on γδT cells.