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目的研究动脉粥样硬化性脑梗死(ACI)发病与低密度脂蛋白(LDL)亚组分颗粒直径大小之间的关系。方法梯度密度超速离心32例ACI患者及32例对照者的血浆LDL,采用2.5%～16.0%聚丙烯酰胺凝胶梯度电泳分析LDL颗粒直径、分布及电泳图形。结果①ACI组三酰甘油(TG),总胆固醇(TC),低密度脂蛋白胆固醇(LDL-C)及载脂蛋白B(ApoB)较对照组明显升高(t=4.03～8.37,P<0.01)。②ACI组LDL颗粒直径犤(24.8±0.7)nm犦较对照组犤(26.0±0.6)nm犦明显变小(t=4.78,P<0.01);两组间A型、B型的分布不同,B型LDL在ACI组分布明显高于对照组(t=6.80,P<0.05)。③ACI组小而密LDL亚组分相对含量犤(50±10)%犦较对照组犤(17±10)%犦明显增多(t=7.76,P<0.001)。④ACI组LDL亚组分颗粒直径与TG(r=-0.47,P<0.01),TC(r=-0.51,P<0.01),LDL-C(r=-0.36,P<0.05),ApoB(r=-0.46,P<0.05)呈负相关。结论ACI组LDL以小颗粒的B型为主,与ACI发生密切相关,TG水平对LDL颗粒直径大小、组成具有调节作用。
Objective To study the relationship between the incidence of atherosclerotic cerebral infarction (ACI) and the particle diameter of subunit of low density lipoprotein (LDL). Methods The plasma LDL of 32 patients with ACI and 32 controls were evaluated by gradient density ultracentrifugation. The diameters, distributions and electrophoretic patterns of LDL particles were analyzed by 2.5% ~ 16.0% polyacrylamide gel electrophoresis. Results ① The triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and apolipoprotein B (ApoB) in ACI group were significantly higher than those in control group (t = 4.03-8.37, P <0.01 ). ② The diameter of LDL particles in ACI group was significantly lower (= = 4.78, P <0.01) than 对 (26.0 ± 0.6) nm 犤 in control group (.8 (24.8 ± 0.7) nm;) Type LDL in the ACI group was significantly higher than the control group (t = 6.80, P <0.05). (3) The relative content of small and dense LDL sub-fractions in ACI group was significantly higher than that in control group (17 ± 10)% 犤 (t = 7.76, P <0.001). (4) The diameters of LDL sub-components in ACI group were significantly different from those of TG (r = -0.47, P <0.01), TC (r = -0.51, P <0.01) = -0.46, P <0.05) was negatively correlated. Conclusion LDL is mainly small particles of type B in ACI group, which is closely related to ACI. TG level regulates the size and composition of LDL particles.