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目的 生殖支原体（Ｍｇ）是从人体分离到的第１２ 种支原体，并认为与男女性泌尿生殖道的炎性相关。１９８８ 年，国外学者曾从４ 例肺炎患者的咽部分离到Ｍｇ。我们检测了２０ 例正常儿童和６０ 例上呼吸道感染儿童的咽试子标本中的Ｍｇ１６ ＳｒＲＮＡ基因。目的在于探讨Ｍｇ 与儿童上呼吸道感染的关系。方法 本文采用灵敏、特异的套式ＰＣＲ检测技术，并对１例阳性标本和Ｍｇ（Ｇ－３７Ｔ）典型株作了ＤＮＡ测序分析。结果 ６０ 例儿童上呼吸道感染者中有１３ 例Ｍｇ 阳性，其中１ 例经ＤＮＡ测序与Ｍｇ（Ｇ－３７Ｔ）典型株完全一致。结论 Ｍｇ 可能是儿童上呼吸道感染的病因之一
Purpose Mycoplasma genitalium (Mg) is the 12th mycoplasma isolated from the human body and is thought to be associated with the inflammation of the genitourinary tract in both sexes. In 1988, foreign scholars from the pharynx of 4 cases of pneumonia isolated Mg. We examined the Mg16 SrRNA gene in pharyngeal specimens of 20 normal children and 60 children with upper respiratory tract infections. The purpose is to explore the relationship between Mg and childhood upper respiratory tract infection. Methods A sensitive and specific nested PCR technique was used in this study. DNA sequencing analysis was performed on one positive specimen and one typical strain of Mg (G-37T). Results Thirteen of 60 children with upper respiratory tract infection were positive for Mg, of which 1 was identical to the typical strain of Mg (G-37T) by DNA sequencing. Conclusion Mg may be one of the causes of upper respiratory tract infection in children