补肾中药对雄激素致不孕大鼠胰腺及卵巢雄激素受体的影响

来源 :中华中医药杂志 | 被引量 : 0次 | 上传用户:yangyi376
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:探讨补肾中药天癸方对雄激素致不孕大鼠(ASR)促排卵的作用机制。方法:新生SD雌性大鼠随机分为3组,ASR模型组:9日龄SD雌性大鼠皮下注射丙酸睾丸酮;中药组:ASR模型在80日龄起每日灌服天癸方;对照组:9日龄SD雌性大鼠皮下注射中性茶油。在106日龄左右(动情前期),放免法测定血Δ4-雄烯二酮(Δ4-A)、总睾酮(TT)、游离睾酮(FT)、雌二醇(E2)、胰岛素(Ins)和C-肽(C-P),RT-PCR方法检测胰腺及卵巢雄激素受体(AR)mRNA表达水平,并观察中药治疗前后上述指标的变化。结果:ASR模型血Δ4-A、TT、FT、E2、Ins、C-P均显著高于对照组(P<0.05,P<0.01),胰腺、卵巢的AR mRNA表达水平明显升高(P<0.01),中药天癸方治疗后,排卵率为58%,各项血激素及胰腺AR mRNA表达水平明显下降,与对照组比较有显著性差异(P<0.05),卵巢AR mRNA水平与治疗前相比无明显改变。结论:ASR模型血雄激素水平升高,上调胰腺、卵巢的AR mRNA表达,引起高胰岛素血症和无排卵。天癸方可降低血雄激素和胰岛素,使胰腺AR mRNA表达水平下降,纠正ASR模型的内分泌-代谢失调状态,虽然卵巢的AR mRNA表达水平仍较高,但在正常的激素环境下仍出现了排卵。 Objective: To explore the mechanism of tonifying kidney invigorating traditional Chinese medicine on ovulation induced by androgen-sterilized rats (ASR). Methods: Newborn SD female rats were randomly divided into three groups: ASR model group: 9-day-old SD female rats were injected subcutaneously with testosterone propionate; Chinese medicine group: ASR model was given Tianqifang daily from 80 days old; 9-day-old SD female rats were injected subcutaneously with neutral tea oil. At 106 days of age (pre-estrus), serum levels of Δ4-androstenedione (Δ4-A), total testosterone (TT), free testosterone (FT), estradiol (E2), insulin and C-peptide (CP) and RT-PCR were used to detect the mRNA expression of androgen receptor (AR) in the pancreas and ovary. The changes of these indexes were observed before and after the treatment with Chinese medicine. Results: The levels of Δ4-A, TT, FT, E2, Ins and CP in ASR model group were significantly higher than those in control group (P <0.05, P <0.01) (P <0.05). The ovarian AR mRNA level was significantly lower than that before treatment (P <0.05), and the ovulation rate was 58% Significant change. CONCLUSION: The blood levels of androgens in ASR model are up-regulated, and the mRNA expression of AR in pancreas and ovary is increased, causing hyperinsulinemia and anovulation. Tiangui Decoction can lower blood androgens and insulin, reduce the expression of AR mRNA in the pancreas and correct the endocrine-metabolic imbalance of the ASR model. Ovary still displays ovulation even though the mRNA expression level of AR is still high in the ovary .
其他文献
文章首先叙述了回声的产生过程以及影响声音质量的因素,然后分析了回声消除器和抑制器解决回声的原理,最后得出结论,利用VoIP性能管理的架构来解决回声问题是可行的。
有机肥是农村中人畜排泄物和动植物腐烂而积累起来的物质。它含有多种植物可以吸收的营养物质,是农作物的养料。它的作用在于不但可提供养分,还可改善耕层生长环境。具有改善土
文章对IPTV业务运营系统的体系架构进行了介绍,给出了各组成模块的功能说明,并对业务运营系统中的关键技术——内容分发、业务质量保障、EPG、接入认证等进行了阐述和分析。