论文部分内容阅读
苹果(Malus domestica)已经完成全基因组测序并公布全基因组图谱,但是基因注释信息很不完善,因此本试验利用RNA-Seq技术对苹果已注释基因结构进行优化和新转录本预测。以‘富士’花后15、30、45、60 d的果肉为材料提取总RNA,构建文库并使用Illumina双末端测序HiSeq 2500平台进行测序,获得高质量的序列(clean reads)。将得到的结果与苹果‘金冠’参考基因组进行比对,共优化了13 272个已注释基因的结构,优化基因5′端和3′端数目分别为8 843个和8 955个,另外,共鉴定得到1 604个新的转录本,部分基因参与了苹果的转录表达调控、生长调节、氨基酸和糖类等的代谢过程。
Apple (Malus domestica) has completed genome-wide sequencing and published the whole genome map, but the gene annotation information is not perfect, so this study used RNA-Seq technology to optimize apple annotated gene structure and new transcript prediction. Total RNA was extracted from the flesh of ’Fuji’ at 15, 30, 45, 60 d after anthesis to construct a library and sequenced using the Illumina dual-end sequencing HiSeq 2500 platform to obtain clean reads. The obtained results were compared with the ’Golden Delicious’ reference genome of apple and the structure of 13 272 annotated genes was optimized. The number of 5 ’end and 3’ end of the optimized gene was 8 843 and 8 955, respectively. A total of 1 604 new transcripts were identified. Some of them were involved in the transcriptional regulation, growth regulation, metabolism of amino acids and carbohydrates in apple.