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黄酮类化合物具有多羟基结构易被氧化,为了解其羟基被氧化后,是否还具有抑菌、抗癌等多种生物学活性,对芦丁光照处理前后的含量、抗氧化活性(清除DPPH.和还原能力)、分子结构以及抑菌活性进行了比较研究.结果表明,随着光照时间的延长,芦丁含量和抗氧化活性均呈下降趋势,降解反应属于一级反应,半衰期为187.3 d.结构鉴定表明,光照处理芦丁母核结构没有降解,只是A环5、7-OH和B环3′、4′-OH氢解离,芦丁在溶液中以负离子或自由基形式存在.芦丁氧化前后的浓度与抑菌效果均成良好的量效关系,其相关系数分别为0.933 2和0.933 0.但氧化芦丁的EC50值(15.58 g/L)与对照(6.34 g/L)相比差异显著(P<0.01).可见,氧化芦丁的抑菌活性明显降低.这进一步表明,芦丁分子结构的细微改变与体外的抗氧化活性和抑菌活性三者之间的构效关系基本一致.
Flavonoids with polyhydroxy structure are easily oxidized. To understand whether the hydroxyl groups are oxidized, the flavonoids also have antibacterial, anticancer and other biological activities. And reducing ability), molecular structure and antibacterial activity were compared.The results showed that with the prolongation of illumination time, the content and the antioxidant activity of rutin all showed a downward trend, and the degradation reaction was of first order with a half-life of 187.3 d. The structure identification showed that the nuclear structure of rutin did not degrade under light irradiation, only the 5,7-OH of A ring and the 3 ’, 4’-OH hydrogen of B ring dissociated, and rutin existed as negative ions or free radicals in solution. The concentrations before and after the butoxide oxidation and bacteriostasis were in a good dose-effect relationship with correlation coefficients of 0.933 2 and 0.933 0. However, EC50 values of oxidized rutin (15.58 g / L) and control (6.34 g / L) (P <0.01) .It can be seen that the antibacterial activity of rutin reduced significantly.This further shows that the slight changes in the molecular structure of rutin and in vitro antioxidant activity and antibacterial activity between the three structure-activity relationship Basically the same.