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仪器:一台紫外分光光度计,如Perkin-Elmer Lambda5。标准溶液:称取NOVO胰高血糖素标准品(每1 mg含e单位)a mg(约相当于12.50iu),置25 ml的容量瓶中,用0.01 N HCl溶解并稀释至刻度。
Instrument: A UV spectrophotometer, such as Perkin-Elmer Lambda5. Standard solution: Weigh NOVO glucagon standard (e mg per 1 mg) a mg (equivalent to about 12.50iu), placed in a 25 ml volumetric flask, dissolved with 0.01 N HCl and diluted to the mark.