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目的探讨白细胞介素１３（ＩＬ－１３）基因在原发性非Ｉｇ系膜增生性肾炎（ＭｓＰＧＮ）、原发 性局灶节段性肾小球硬化症（ＦＳＧＳ）和狼疮肾炎（ＬＮ）患者肾组织的表达及其意义。方法采用原位 杂交技术（ＩＳＨ）检测３４例上述３种肾脏病患者及４例对照组肾组织中 ＩＬ－１３ ｍＲＮＡ表达量的变化， 分析ＩＬ－１３基因表达量与肾功能变化的关系。结果 ＩＬ－１３ ｍＲＮＡ在ＦＳＧＳ、ＭｓＰＧＮ和ＬＮ患者肾组织 内表达均增高，而对照者肾组织内元明显ＩＬ－１３ ｍＲＮＡ表达。ＬＮ患者肾小管间质区ＩＬ－１３ ｍＲＮＡ表达 水平与狼疮活动相关， ＭｓＰＧＮ及ＦＳＧＳ患者肾小管间质区 ＩＬ－１３ ｍＲＮＡ表达量与肾功能变化及２４ ｈ 尿蛋白量相关。结论ＩＬ－１３可能直接参与人类肾小球疾病的免疫反应过程。
Objective To investigate the clinical significance of interleukin 13 (IL-13) gene expression in patients with primary non-Ig mesangial proliferative glomerulonephritis (MsPGN), primary focal segmental glomerulosclerosis (FSGS) and lupus nephritis Renal Tissue Expression and Its Significance. Methods In situ hybridization (ISH) was used to detect the changes of IL-13 mRNA expression in renal tissues of 34 patients with above three kinds of nephropathy and 4 controls. The relationship between IL-13 gene expression and renal function was analyzed. Results The expression of IL-13 mRNA in renal tissues of FSGS, MsPGN and LN patients were increased, while the expression of IL-13 mRNA in renal tissues was significantly increased in IL-13 mRNA. The expression of IL-13 mRNA in tubulointerstitial region of LN patients was correlated with lupus activity. The expression of IL-13 mRNA in tubulointerstitial region of MsPGN and FSGS patients was correlated with renal function and 24-hour urinary protein. Conclusion IL-13 may be directly involved in the immune response of human glomerular diseases.