[Objective] To study the effects of different hormones the suspension cell growth and development of Bupleurum falcatum L.. [Method] The plant growth hormone of medium for Bupleurum falcatum L. suspension cell cultures was optimized by the test method of uniform design. [Result] The optimal hormone formulation of B. falcatum suspension cell cultures was N6 + 0.5 mg/L NAA + 0.75 mg/L 6-BA + 1.5 mg/L KT. Based on the optimal hormone formulation to fit the curves about the relationship of the cell fresh-weight t and the cell dry-weight in incubation time, the fitting equation of the relationship between the cell fresh-weight and the incubation time was Y=5.703 6X2-41.429X+136.44; the fitting equation of the relationship between the cell dry-weight and the incubation time was Y=2.021 8X2-22.595X+57.19. [Conclusion] This study can lay the foundation for the further study and the large-scale culture about the B. falcatum. [Objective] To study the effects of different hormones the suspension cell growth and development of Bupleurum falcatum L .. [Method] The plant growth hormone of medium for Bupleurum falcatum L. suspension cell cultures was optimized by the test method of uniform design. [ Result] The optimal hormone formulation of B. falcatum suspension cell cultures was N6 + 0.5 mg / L NAA + 0.75 mg / L 6-BA + 1.5 mg / L KT. Based on the optimal hormone formulation to fit the curves about the relationship of the cell fresh-weight t and the cell dry-weight in incubation time, the fitting equation of the relationship between the cell fresh-weight and the incubation time was Y = 5.703 6X2-41.429X + 136.44; the fitting equation of the relationship between the cell dry-weight and the incubation time was Y = 2.021 8X2-22.595X + 57.19. [Conclusion] This study can lay the foundation for the further study and the large-scale culture about the B. falcatum.