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为研究二甲基甲酰胺（ＤＭＦ）的免疫毒性，从免疫器官病理组织学检查、足垫肿胀实验、外周血淋巴细胞α－醋酸萘酯酶（ＡＮＡＥ）染色，脾细胞Ⅱ型白细胞介素（ＩＬ－２）释放，体外刀豆素Ａ（ＣｏｎＡ）诱导的淋巴细胞增殖等四个方面研究了ＤＭＦ对动物免疫器官及细胞介导免疫的影响。结果显示，ＤＭＦ可使脾脏、胸腺相对重量减轻，绵羊红细胞介导的足垫反应值明显降低。皮下注射及灌胃染毒可使不同种属动物的ＡＮＡＥ阳性率降低，在１／１０～１／１６０ＬＤ５０情况下还可使ＣｏｎＡ诱导的ＩＬ－２释放明显减少。体外试验显示，ＤＭＦ在１０～１５０ｍｍｏｌ／Ｌ之间可明显抑制ＣｏｎＡ诱导的淋巴细胞增殖，表明ＤＭＦ对小鼠具有细胞免疫毒性作用。
In order to study the immunotoxicity of dimethylformamide (DMF), immunofluorescence histopathology, footpad swelling test, ANAE staining of peripheral blood lymphocytes, type II interleukin (IL- IL-2 release, and ConA-induced lymphocyte proliferation in vitro. The effects of DMF on immune organs and cell-mediated immunity were studied. The results showed that DMF can reduce the relative weight of the spleen and thymus, and reduce the erythrocyte-mediated footpad response. Subcutaneous injection and intragastric administration can reduce the positive rate of ANAE in different species of animals, and significantly reduce the release of ConA-induced IL-2 in the range of 1 / 10-1 / 160LD50. In vitro experiments show that DMF between 10 ~ 150mmol / L ConA-induced lymphocyte proliferation can be significantly inhibited, indicating that mice with murine cellular immunotoxicity.