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目的探讨四君子汤保护骨损伤小鼠细菌易位及对骨修复的促进作用。方法在100只健康小鼠中选取10只为对照组,其余90只小鼠造模后随机分为中药组、抗生素组、自然恢复组(各30只)。抗生素组小鼠每天腹腔内注射青霉素和链霉素,中药组在此基础上给予四君子汤灌胃,自然恢复组以0.9%Na Cl溶液灌胃。造模10天后,检测肝脏细菌易位及巨噬细胞吞噬功能;观察中药组、抗生素组和自然恢复组骨折后2周、4周、6周和8周骨痂面积及骨体积分数(BVF)。结果中药组小鼠肝脏细菌易位率和肝脏活菌计数均显著低于自然恢复组和抗生素组(P<0.01);中药组巨噬细胞吞噬能力显著高于自然恢复组和抗生素组(P<0.01);中药组骨痂面积缩小最快(P<0.01);在不同时间点,中药组BVF均高于自然恢复组和抗生素组(P<0.01)。结论四君子汤能够抑制骨损伤小鼠细菌易位、提高巨噬细胞的吞噬功能以促进骨折修复。
Objective To investigate the effect of Sijunzi Decoction on bacterial translocation in mice with bone injury and its effect on bone repair. Methods Ten healthy mice were selected as the control group. The remaining 90 mice were randomly divided into TCM group, antibiotic group and natural recovery group (30 rats in each group). The mice in the antibiotic group were injected with penicillin and streptomycin intraperitoneally every day. On the basis of this, the traditional Chinese medicine group was administered with Sijunzi Decoction, and the natural recovery group was orally administered with 0.9% NaCl solution. 10 days after model establishment, liver bacterial translocation and phagocytosis of macrophages were detected. The callus area and bone volume fraction (BVF) were observed at 2 weeks, 4 weeks, 6 weeks and 8 weeks after fracture in the traditional Chinese medicine group, antibiotic group and natural recovery group. . Results The liver bacterial translocation rate and viable count of liver in TCM group were significantly lower than those in natural recovery group and antibiotic group (P <0.01). The macrophage phagocytic capacity in TCM group was significantly higher than that in natural recovery group and antibiotic group (P < 0.01). The callus area of TCM group contracted the fastest (P <0.01). At different time points, BVF of TCM group was higher than that of natural recovery group and antibiotic group (P <0.01). Conclusion Sijunzi Decoction can inhibit bacterial translocation in mice with bone injury and improve phagocytosis of macrophages to promote fracture healing.