本文介绍了血小板释放TXB_2的酶免疫分析法。用混合酸酐法交联β-半乳糖苷酶与TXB_2。通过双抗体沉淀法分离免疫复合物与游离TXB_2。以4-甲基伞形酮-β-D-半乳糖苷作底物测定酶促反应产物的荧光强度。该方法TXB_2最低检测值<0.008pmol(<3.125pg),批内变异系数为5.9％,批间变异系数为5.4％。酶免疫分析法与放射免疫分析法测定血小板样品进行比较,直线相关系数r=0.955,回归方程Y=0.811x+1.397。 This article describes the enzyme immunoassay for the release of TXB_2 from platelets. Cross-linking of β-galactosidase with TXB_2 by mixed anhydride method. Immune complexes and free TXB_2 were isolated by double antibody precipitation. The fluorescence intensity of the enzymatic reaction product was measured using 4-methylumbelliferyl-β-D-galactoside as a substrate. The minimum detectable value of TXB_2 was less than 0.008 pmol (<3.125 pg), the intra-assay CV was 5.9% and the inter-assay CV was 5.4%. Enzyme immunoassay and radioimmunoassay platelet samples for comparison, the linear correlation coefficient r = 0.955, the regression equation Y = 0.811x + 1.397.