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目的 观察6- 羟多巴胺毁损的帕金森病(Parkinson’sdisease, PD)模型大鼠脚桥核(pedunculopontinenu cleus, PPN)神经元放电频率和放电形式的变化。方法 采用在体玻璃微电极细胞外记录法,记录正常对照组和PD模型组大鼠PPN神经元的电活动。结果 对照组和PD组大鼠PPN神经元的放电频率分别为(9. 0±0. 8)Hz[ (0. 5 25. 2)Hz, n=56]和(16. 1±1. 6)Hz[ (1. 2 49. 7)Hz, n=57],PD组大鼠的放电频率显著高于对照组(P<0. 001)。在对照组大鼠脚桥核, 68% (38 /56)的神经元呈现规则放电, 27% (15 /56)呈现不规则放电, 5% (3 /56)为爆发式放电;在PD组大鼠脚桥核,具有规则、不规则和爆发式放电的神经元比例分别为39% (22 /57)、47%(27 /57)和14% (8 /57),PD组大鼠具有不规则放电的神经元比例明显高于对照组(P<0. 05)。结论 PD大鼠PPN中神经元的放电频率增高和不规则放电增多, 这可能在帕金森病的病理生理变化中具有重要作用。
Objective To observe the changes of discharge frequency and discharge pattern of pedunculopontinenu cleus (PPN) neurons in 6-hydroxydopamine-lesioned Parkinson’s disease (PD) model rats. Methods In vivo glass microelectrode extracellular recording method was used to record the electrical activity of PPN neurons in normal control group and PD model group. Results The discharge frequency of PPN neurons in control group and PD group were (9. 0 ± 0. 8) Hz [(0. 5 25.2) Hz, n = 56] and (16.1 ± 1.6 ) Hz [(1.249.7) Hz, n = 57]. The discharge frequency in PD group was significantly higher than that in control group (P <0.001). In control group, 68% (38/56) neurons showed regular discharge in the foot bridge nucleus, 27% (15/56) showed irregular discharge and 5% (3/56) burst discharge. In PD group The proportion of neurons with regular, irregular and explosive discharge in rat foot bridge nucleus was 39% (22/57), 47% (27/57) and 14% (8/57) respectively. PD rats had The proportion of irregular discharge neurons was significantly higher than that of the control group (P <0.05). Conclusion The discharge frequency and irregular discharge of neurons in PD rats are increased, which may play an important role in the pathophysiological changes of Parkinson’s disease.