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促炎细胞因子白细胞介素－ １β和肿瘤坏死因子－ α是血管平滑肌细胞的强效促分裂原，为了探讨其促血管平滑肌细胞增殖是否与细胞外基质降解及粘附蛋白合成有关，本文以体外培养的血管平滑肌细胞为研究对象，应用Ｎｏｒｔｈｅｒｎ 印迹及基质金属蛋白酶－ ２ 活性酶图分析方法动态观察白细胞介素－ １β、肿瘤坏死因子－ α对血管平滑肌细胞表达基质金属蛋白酶－２ 及骨桥蛋白的影响。结果发现，白细胞介素－１β和肿瘤坏死因子－ α均可显著诱导基质金属蛋白酶－ ２ 及骨桥蛋白的基因表达，血管平滑肌细胞受两种细胞因子刺激１２ ｈ 后，基质金属蛋白酶－２ 及骨桥蛋白ｍＲＮＡ表达活性最高，分别达到对照细胞的３ 倍左右和１０ 倍以上。对细胞培养基基质金属蛋白酶活性进行酶图分析的结果发现，肿瘤坏死因子－α及白细胞介素－１β作用于血管平滑肌细胞１２ 及２４ ｈ 时，基质金属蛋白酶降解明胶的活性约为对照细胞培养基的２ 倍和１．５ 倍。提示这类细胞因子可同时在多位点上对血管平滑肌细胞的迁移与增殖发挥促进作用。
The proinflammatory cytokines interleukin - 1β and tumor necrosis factor - α are potent mitogens of vascular smooth muscle cells. In order to investigate whether the proliferation of vascular smooth muscle cells is related to the degradation of extracellular matrix and the synthesis of adhesion protein, Cultured vascular smooth muscle cells were used as experimental subjects. Northern blotting and MMP - 2 activity assay were used to observe the effect of interleukin - 1β and tumor necrosis factor - α on the expression of matrix metalloproteinase - 2 and osteopontin in vascular smooth muscle cells Impact. The results showed that interleukin - 1β and tumor necrosis factor - α significantly induced gene expression of MMP - 2 and osteopontin. After vascular smooth muscle cells were stimulated by two cytokines for 12 h, matrix metalloproteinase - 2 Osteopontin mRNA expression activity of the highest, respectively, to control cells about 3 times and 10 times. Enzyme-linked assay of matrix metalloproteinase activity in cell culture medium revealed that the activity of matrix metalloproteinase-degrading gelatin was about the control cell culture when tumor necrosis factor-α and interleukin-1β were applied to vascular smooth muscle cells at 12 and 24 h Base 2 times and 1.5 times. These cytokines could promote the migration and proliferation of vascular smooth muscle cells at multiple sites at the same time.